Home Crop Monitoring Tomatoes with enhanced antioxidant properties created with genetic engineering

Tomatoes with enhanced antioxidant properties created with genetic engineering


The College of Organic Sciences, College of Science, the College of Hong Kong (HKU), in collaboration with the Institut de Biologie Moléculaire des Plantes (CNRS, Strasbourg, France), has recognized a brand new technique to concurrently improve health-promoting vitamin E by ~6-fold and double each provitamin A and lycopene contents in tomatoes, to considerably increase antioxidant properties.

The analysis group manipulated the plant isoprenoid pathway by means of the utilization of a variant of 3-hydroxy-3-methylglutaryl-coenzyme A synthase (HMGS). The overexpression of HMGS in tomatoes elevated not solely phytosterols, squalene, provitamin A and lycopene, but in addition vitamin E (α-tocopherol) by 494%.

The HMGS DNA utilized in these experiments originated from a meals crop, Brassica juncea (Indian mustard), that yields edible leaves, stems and seeds, the latter utilized in vegetable oil manufacturing.

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Earlier, this analysis group reported that the recombinant HMGS variant S359A (during which amino acid residue “serine” at place 359 was switched to “alanine”) displays 10-fold larger enzyme exercise. The introduction of S359A within the mannequin plant Arabidopsis elevated phytosterol content material.

Now, the analysis group has launched the S359A into tomatoes, a crop plant. Though there have been no variations within the look and dimension of the remodeled tomato fruits, whole carotenoids together with provitamin A and lycopene elevated drastically by 169% and 111%, respectively, as noticed by a deeper color of carotenoid extracts in S359A tomatoes over the management.

Moreover, these carotenoid extracts exhibited 89.5-96.5% larger antioxidant exercise than the management. Apart from carotenoids, the remodeled tomatoes displayed elevations in vitamin E (α-tocopherol, 494%), squalene (210%), and phytosterols (94%). These observations had been attributed to the elevated expression of genes within the isoprenoid pathway.

Professor Chye Mee-len who led this analysis stated: “Increasing health-promoting components in crops is an important research area that aligns with the aspirations of Dr Wilson and Mrs Amelia Wong on the use of plant biotechnology for a sustainable future.

The accumulation of the healthy components in food crops would provide added-value to fruits and vegetables in the human diet, as well as enrich feed for livestock and aquaculture.” Dr Wang Mingfu added: “Extracts with enriched phytosterols, vitamin E and carotenoids can be utilized within the manufacturing of anti-aging cream and sun-care lotion. These compounds present glorious anti-inflammatory and antioxidant exercise.

Story Supply: Supplies offered by The College of Hong Kong.

Read next: what makes a good tomato planter machine?

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